introduction required
online booking
About this instrument
The controller can be used for single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L. The current configuration is optimised for cell culture and not suitable for bacterial cultures. For bacterial cultures, see here. This article describes the use of the 3l single use reactors. For the 250 ml MiniBio please check here.
Compatible single-use bioreactors are AppliFlexST (Applikon) or CellReady 3l (Merck).
- 3 channel MFC for precise control of Oxygen, Nitrogen and CO2
- 1 valve control for air overlay
- four peristaltic pumps for adding e.g. base and glucose
- Sensors for dissolved oxygen (DO), pH and temperature
- heating blanket for temperature control
- Lucullus LITE is provided for data logging
Hot to get access
Help and manuals
Workflow overview & check list
- Preparation
- perform two point calibration (pH4 and pH7) of pH sensor
- autoclave pH and DO sensors, liquid addition bottles and tubes
- Polarisation of DO sensor
- fill reaktor vessel with medium (laf bench)
- connect reactor to controller
- air overlay on
- heating blanket on (20%)
- stirrer set on low
- pH sensor can be connected but on hold
- temperature sensor on (fill tube with water)
- leave for least 4h or over night
- perform one-point calibration of DO = 100%
- check pH of medium (be sure that medium tolerates pH)
- Inoculation & start
- connect liquid addition bottles and prime the pumps
- start data logging in lucullus
- move reactor including pH and DO sensors and liquid addition bottles to laf bench
- inoculate with syringe
- reconnect sensors cables and peristaltic pumps
- mount off-gas filter heating
- In Web UI, start all controllers and off gas filter heating
- add a comment in Lucullus ‘inoculation’ to generate time stamp